merquaco.pixel_classification.generate_detachment_mask

merquaco.pixel_classification.generate_detachment_mask(transcripts_mask_path: str | Path, dapi_mask_path: str | Path, detachment_mask_path: str | Path) → ndarray

Generate and save gel detachment mask by subtracting transcript mask from DAPI mask

Parameters:

• transcripts_mask_path (str or Path) – Path to binary transcript mask

• dapi_mask_path (str or Path) – Path to binary DAPI mask

• detachment_mask_path (str or Path) – Path at which to save gel detachment mask

Returns:

detachment_mask – Detachment mask binary array

Return type:

np.ndarray